Exome resequencing and GWAS for growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa.
Identifieur interne : 000970 ( Main/Exploration ); précédent : 000969; suivant : 000971Exome resequencing and GWAS for growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa.
Auteurs : Fernando P. Guerra [États-Unis, Chili] ; Haktan Suren [États-Unis] ; Jason Holliday [États-Unis] ; James H. Richards [États-Unis] ; Oliver Fiehn [États-Unis] ; Randi Famula [États-Unis] ; Brian J. Stanton [États-Unis] ; Richard Shuren [États-Unis] ; Robert Sykes [États-Unis] ; Mark F. Davis [États-Unis] ; David B. Neale [États-Unis]Source :
- BMC genomics [ 1471-2164 ] ; 2019.
Descripteurs français
- KwdFr :
- Azote (métabolisme), Bois (génétique), Bois (métabolisme), Californie (MeSH), Caractère quantitatif héréditaire (MeSH), Carbone (métabolisme), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Génome végétal (MeSH), Lignine (biosynthèse), Marqueurs génétiques (MeSH), Métabolome (MeSH), Polymorphisme de nucléotide simple (MeSH), Populus (génétique), Populus (métabolisme), Séquençage de l'exome entier (MeSH), Voies et réseaux métaboliques (génétique), Étude d'association pangénomique (MeSH).
- MESH :
- biosynthèse : Lignine.
- génétique : Bois, Feuilles de plante, Populus, Voies et réseaux métaboliques.
- métabolisme : Azote, Bois, Carbone, Feuilles de plante, Populus.
- Californie, Caractère quantitatif héréditaire, Génome végétal, Marqueurs génétiques, Métabolome, Polymorphisme de nucléotide simple, Séquençage de l'exome entier, Étude d'association pangénomique.
English descriptors
- KwdEn :
- California (MeSH), Carbon (metabolism), Genetic Markers (MeSH), Genome, Plant (MeSH), Genome-Wide Association Study (MeSH), Lignin (biosynthesis), Metabolic Networks and Pathways (genetics), Metabolome (MeSH), Nitrogen (metabolism), Plant Leaves (genetics), Plant Leaves (metabolism), Polymorphism, Single Nucleotide (MeSH), Populus (genetics), Populus (metabolism), Quantitative Trait, Heritable (MeSH), Whole Exome Sequencing (MeSH), Wood (genetics), Wood (metabolism).
- MESH :
- chemical , biosynthesis : Lignin.
- chemical , metabolism : Carbon, Nitrogen.
- geographic : California, Genetic Markers.
- genetics : Metabolic Networks and Pathways, Plant Leaves, Populus, Wood.
- metabolism : Plant Leaves, Populus, Wood.
- Genome, Plant, Genome-Wide Association Study, Metabolome, Polymorphism, Single Nucleotide, Quantitative Trait, Heritable, Whole Exome Sequencing.
Abstract
BACKGROUND
Populus trichocarpa is an important forest tree species for the generation of lignocellulosic ethanol. Understanding the genomic basis of biomass production and chemical composition of wood is fundamental in supporting genetic improvement programs. Considerable variation has been observed in this species for complex traits related to growth, phenology, ecophysiology and wood chemistry. Those traits are influenced by both polygenic control and environmental effects, and their genome architecture and regulation are only partially understood. Genome wide association studies (GWAS) represent an approach to advance that aim using thousands of single nucleotide polymorphisms (SNPs). Genotyping using exome capture methodologies represent an efficient approach to identify specific functional regions of genomes underlying phenotypic variation.
RESULTS
We identified 813 K SNPs, which were utilized for genotyping 461 P. trichocarpa clones, representing 101 provenances collected from Oregon and Washington, and established in California. A GWAS performed on 20 traits, considering single SNP-marker tests identified a variable number of significant SNPs (p-value < 6.1479E-8) in association with diameter, height, leaf carbon and nitrogen contents, and δ
CONCLUSIONS
SNP-markers within genes associated with traits of importance for biomass production were detected. They contribute to characterize the genomic architecture of P. trichocarpa biomass required to support the development and application of marker breeding technologies.
DOI: 10.1186/s12864-019-6160-9
PubMed: 31747881
PubMed Central: PMC6864938
Affiliations:
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Stanton</name><affiliation wicri:level="1"><nlm:affiliation>Biological Research Group, GreenWood Resources, Portland, OR, 97201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Research Group, GreenWood Resources, Portland, OR, 97201</wicri:regionArea><wicri:noRegion>97201</wicri:noRegion></affiliation></author><author><name sortKey="Shuren, Richard" sort="Shuren, Richard" uniqKey="Shuren R" first="Richard" last="Shuren">Richard Shuren</name><affiliation wicri:level="1"><nlm:affiliation>Biological Research Group, GreenWood Resources, Portland, OR, 97201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Research Group, GreenWood Resources, Portland, OR, 97201</wicri:regionArea><wicri:noRegion>97201</wicri:noRegion></affiliation></author><author><name sortKey="Sykes, Robert" sort="Sykes, Robert" uniqKey="Sykes R" first="Robert" last="Sykes">Robert Sykes</name><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. 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Neale</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA. dbneale@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Bioenergy Research Center, University of California at Davis, Davis, CA, 95616, USA. dbneale@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Bioenergy Research Center, University of California at Davis, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31747881</idno><idno type="pmid">31747881</idno><idno type="doi">10.1186/s12864-019-6160-9</idno><idno type="pmc">PMC6864938</idno><idno type="wicri:Area/Main/Corpus">000604</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000604</idno><idno type="wicri:Area/Main/Curation">000604</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000604</idno><idno type="wicri:Area/Main/Exploration">000604</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Exome resequencing and GWAS for growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa.</title><author><name sortKey="Guerra, Fernando P" sort="Guerra, Fernando P" uniqKey="Guerra F" first="Fernando P" last="Guerra">Fernando P. Guerra</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencias Biológicas, Universidad de Talca, Talca, P.O. Box 747, 3460000, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Instituto de Ciencias Biológicas, Universidad de Talca, Talca, P.O. Box 747, 3460000</wicri:regionArea><wicri:noRegion>3460000</wicri:noRegion></affiliation></author><author><name sortKey="Suren, Haktan" sort="Suren, Haktan" uniqKey="Suren H" first="Haktan" last="Suren">Haktan Suren</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061</wicri:regionArea><wicri:noRegion>24061</wicri:noRegion></affiliation></author><author><name sortKey="Holliday, Jason" sort="Holliday, Jason" uniqKey="Holliday J" first="Jason" last="Holliday">Jason Holliday</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061</wicri:regionArea><wicri:noRegion>24061</wicri:noRegion></affiliation></author><author><name sortKey="Richards, James H" sort="Richards, James H" uniqKey="Richards J" first="James H" last="Richards">James H. Richards</name><affiliation wicri:level="1"><nlm:affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author><author><name sortKey="Fiehn, Oliver" sort="Fiehn, Oliver" uniqKey="Fiehn O" first="Oliver" last="Fiehn">Oliver Fiehn</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular and Cellular Biology & Genome Center, University of California, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Biology & Genome Center, University of California, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author><author><name sortKey="Famula, Randi" sort="Famula, Randi" uniqKey="Famula R" first="Randi" last="Famula">Randi Famula</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author><author><name sortKey="Stanton, Brian J" sort="Stanton, Brian J" uniqKey="Stanton B" first="Brian J" last="Stanton">Brian J. Stanton</name><affiliation wicri:level="1"><nlm:affiliation>Biological Research Group, GreenWood Resources, Portland, OR, 97201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Research Group, GreenWood Resources, Portland, OR, 97201</wicri:regionArea><wicri:noRegion>97201</wicri:noRegion></affiliation></author><author><name sortKey="Shuren, Richard" sort="Shuren, Richard" uniqKey="Shuren R" first="Richard" last="Shuren">Richard Shuren</name><affiliation wicri:level="1"><nlm:affiliation>Biological Research Group, GreenWood Resources, Portland, OR, 97201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Research Group, GreenWood Resources, Portland, OR, 97201</wicri:regionArea><wicri:noRegion>97201</wicri:noRegion></affiliation></author><author><name sortKey="Sykes, Robert" sort="Sykes, Robert" uniqKey="Sykes R" first="Robert" last="Sykes">Robert Sykes</name><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Neale, David B" sort="Neale, David B" uniqKey="Neale D" first="David B" last="Neale">David B. Neale</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA. dbneale@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Bioenergy Research Center, University of California at Davis, Davis, CA, 95616, USA. dbneale@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Bioenergy Research Center, University of California at Davis, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>California (MeSH)</term><term>Carbon (metabolism)</term><term>Genetic Markers (MeSH)</term><term>Genome, Plant (MeSH)</term><term>Genome-Wide Association Study (MeSH)</term><term>Lignin (biosynthesis)</term><term>Metabolic Networks and Pathways (genetics)</term><term>Metabolome (MeSH)</term><term>Nitrogen (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Polymorphism, Single Nucleotide (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Quantitative Trait, Heritable (MeSH)</term><term>Whole Exome Sequencing (MeSH)</term><term>Wood (genetics)</term><term>Wood (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Azote (métabolisme)</term><term>Bois (génétique)</term><term>Bois (métabolisme)</term><term>Californie (MeSH)</term><term>Caractère quantitatif héréditaire (MeSH)</term><term>Carbone (métabolisme)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Génome végétal (MeSH)</term><term>Lignine (biosynthèse)</term><term>Marqueurs génétiques (MeSH)</term><term>Métabolome (MeSH)</term><term>Polymorphisme de nucléotide simple (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Séquençage de l'exome entier (MeSH)</term><term>Voies et réseaux métaboliques (génétique)</term><term>Étude d'association pangénomique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Nitrogen</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>California</term><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Metabolic Networks and Pathways</term><term>Plant Leaves</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bois</term><term>Feuilles de plante</term><term>Populus</term><term>Voies et réseaux métaboliques</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Bois</term><term>Carbone</term><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genome, Plant</term><term>Genome-Wide Association Study</term><term>Metabolome</term><term>Polymorphism, Single Nucleotide</term><term>Quantitative Trait, Heritable</term><term>Whole Exome Sequencing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Californie</term><term>Caractère quantitatif héréditaire</term><term>Génome végétal</term><term>Marqueurs génétiques</term><term>Métabolome</term><term>Polymorphisme de nucléotide simple</term><term>Séquençage de l'exome entier</term><term>Étude d'association pangénomique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Populus trichocarpa is an important forest tree species for the generation of lignocellulosic ethanol. Understanding the genomic basis of biomass production and chemical composition of wood is fundamental in supporting genetic improvement programs. Considerable variation has been observed in this species for complex traits related to growth, phenology, ecophysiology and wood chemistry. Those traits are influenced by both polygenic control and environmental effects, and their genome architecture and regulation are only partially understood. Genome wide association studies (GWAS) represent an approach to advance that aim using thousands of single nucleotide polymorphisms (SNPs). Genotyping using exome capture methodologies represent an efficient approach to identify specific functional regions of genomes underlying phenotypic variation.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>We identified 813 K SNPs, which were utilized for genotyping 461 P. trichocarpa clones, representing 101 provenances collected from Oregon and Washington, and established in California. A GWAS performed on 20 traits, considering single SNP-marker tests identified a variable number of significant SNPs (p-value < 6.1479E-8) in association with diameter, height, leaf carbon and nitrogen contents, and δ</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>SNP-markers within genes associated with traits of importance for biomass production were detected. They contribute to characterize the genomic architecture of P. trichocarpa biomass required to support the development and application of marker breeding technologies.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31747881</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>02</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Nov</Month><Day>20</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Exome resequencing and GWAS for growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa.</ArticleTitle><Pagination><MedlinePgn>875</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-019-6160-9</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Populus trichocarpa is an important forest tree species for the generation of lignocellulosic ethanol. Understanding the genomic basis of biomass production and chemical composition of wood is fundamental in supporting genetic improvement programs. Considerable variation has been observed in this species for complex traits related to growth, phenology, ecophysiology and wood chemistry. Those traits are influenced by both polygenic control and environmental effects, and their genome architecture and regulation are only partially understood. Genome wide association studies (GWAS) represent an approach to advance that aim using thousands of single nucleotide polymorphisms (SNPs). Genotyping using exome capture methodologies represent an efficient approach to identify specific functional regions of genomes underlying phenotypic variation.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We identified 813 K SNPs, which were utilized for genotyping 461 P. trichocarpa clones, representing 101 provenances collected from Oregon and Washington, and established in California. A GWAS performed on 20 traits, considering single SNP-marker tests identified a variable number of significant SNPs (p-value < 6.1479E-8) in association with diameter, height, leaf carbon and nitrogen contents, and δ<sup>15</sup>N. The number of significant SNPs ranged from 2 to 220 per trait. Additionally, multiple-marker analyses by sliding-windows tests detected between 6 and 192 significant windows for the analyzed traits. The significant SNPs resided within genes that encode proteins belonging to different functional classes as such protein synthesis, energy/metabolism and DNA/RNA metabolism, among others.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">SNP-markers within genes associated with traits of importance for biomass production were detected. They contribute to characterize the genomic architecture of P. trichocarpa biomass required to support the development and application of marker breeding technologies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guerra</LastName><ForeName>Fernando P</ForeName><Initials>FP</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Instituto de Ciencias Biológicas, Universidad de Talca, Talca, P.O. Box 747, 3460000, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suren</LastName><ForeName>Haktan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Holliday</LastName><ForeName>Jason</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Richards</LastName><ForeName>James H</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fiehn</LastName><ForeName>Oliver</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cellular Biology & Genome Center, University of California, Davis, CA, 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Famula</LastName><ForeName>Randi</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stanton</LastName><ForeName>Brian J</ForeName><Initials>BJ</Initials><AffiliationInfo><Affiliation>Biological Research Group, GreenWood Resources, Portland, OR, 97201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shuren</LastName><ForeName>Richard</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Biological Research Group, GreenWood Resources, Portland, OR, 97201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sykes</LastName><ForeName>Robert</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>Mark F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neale</LastName><ForeName>David B</ForeName><Initials>DB</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, University of California at Davis, 262C Robbins Hall, Mail Stop 4, Davis, CA, 95616, USA. dbneale@ucdavis.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Bioenergy Research Center, University of California at Davis, Davis, CA, 95616, USA. dbneale@ucdavis.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2011-68005-30407</GrantID><Agency>USDA National Institute of Food and Agriculture</Agency><Country></Country></Grant><Grant><GrantID>1054444</GrantID><Agency>National Science Foundation Plant Genome Research Program</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002140" MajorTopicYN="N" Type="Geographic">California</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="Y">Genome, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055106" MajorTopicYN="N">Genome-Wide Association Study</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053858" MajorTopicYN="N">Metabolic Networks and Pathways</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055442" MajorTopicYN="N">Metabolome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020641" MajorTopicYN="N">Polymorphism, Single Nucleotide</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019655" MajorTopicYN="Y">Quantitative Trait, Heritable</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000073359" MajorTopicYN="N">Whole Exome Sequencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cellulose</Keyword><Keyword MajorTopicYN="N">GWAS</Keyword><Keyword MajorTopicYN="N">Growth</Keyword><Keyword 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IdType="doi">10.1186/s12864-019-6160-9</ArticleId><ArticleId IdType="pii">10.1186/s12864-019-6160-9</ArticleId><ArticleId IdType="pmc">PMC6864938</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Stat Med. 2011 May 10;30(10):1157-78</Citation><ArticleIdList><ArticleId IdType="pubmed">21337593</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2009 Jul 15;25(14):1754-60</Citation><ArticleIdList><ArticleId IdType="pubmed">19451168</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol J. 2015 Apr;10(4):510-24</Citation><ArticleIdList><ArticleId IdType="pubmed">25676392</ArticleId></ArticleIdList></Reference><Reference><Citation>Genet Epidemiol. 2006 Nov;30(7):627-35</Citation><ArticleIdList><ArticleId IdType="pubmed">16858698</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Nov;200(3):727-42</Citation><ArticleIdList><ArticleId IdType="pubmed">23889128</ArticleId></ArticleIdList></Reference><Reference><Citation>Comput Stat Data Anal. 2009 Mar 15;53(5):1794-1801</Citation><ArticleIdList><ArticleId IdType="pubmed">20161066</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Nov;200(3):710-26</Citation><ArticleIdList><ArticleId IdType="pubmed">23889164</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2014 Oct;46(10):1089-96</Citation><ArticleIdList><ArticleId IdType="pubmed">25151358</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2011 Mar;18(3):270-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21297639</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2017 Jan 12;541(7636):212-216</Citation><ArticleIdList><ArticleId IdType="pubmed">28024298</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2014 Mar;201(4):1263-76</Citation><ArticleIdList><ArticleId IdType="pubmed">24491114</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2010 Sep;20(9):1297-303</Citation><ArticleIdList><ArticleId IdType="pubmed">20644199</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Feb;197(3):777-90</Citation><ArticleIdList><ArticleId IdType="pubmed">23278123</ArticleId></ArticleIdList></Reference><Reference><Citation>Physiol Plant. 2010 Dec;140(4):334-41</Citation><ArticleIdList><ArticleId IdType="pubmed">20738805</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA. 2011 Mar;17(3):501-11</Citation><ArticleIdList><ArticleId IdType="pubmed">21224377</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2007 Oct 1;23(19):2633-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17586829</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2014 May;23(10):2486-99</Citation><ArticleIdList><ArticleId IdType="pubmed">24750333</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2017 Jul 11;18(1):524</Citation><ArticleIdList><ArticleId IdType="pubmed">28693539</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2017 Dec 19;8:2164</Citation><ArticleIdList><ArticleId IdType="pubmed">29312405</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Dec 14;13:703</Citation><ArticleIdList><ArticleId IdType="pubmed">23241106</ArticleId></ArticleIdList></Reference><Reference><Citation>Photosynth Res. 2014 Feb;119(1-2):119-29</Citation><ArticleIdList><ArticleId IdType="pubmed">23893317</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2009 Nov;29(11):1329-39</Citation><ArticleIdList><ArticleId IdType="pubmed">19773340</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2018 Oct;220(1):300-316</Citation><ArticleIdList><ArticleId IdType="pubmed">29963703</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2019 Jul;223(1):293-309</Citation><ArticleIdList><ArticleId IdType="pubmed">30843213</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2009 Apr;58(2):208-19</Citation><ArticleIdList><ArticleId IdType="pubmed">19077168</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Hum Genet. 2011 Jan 7;88(1):76-82</Citation><ArticleIdList><ArticleId IdType="pubmed">21167468</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2011 May;43(5):491-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21478889</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11479-84</Citation><ArticleIdList><ArticleId IdType="pubmed">11562485</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2009 Feb;27(2):182-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19182786</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2014 Dec;23(23):5771-90</Citation><ArticleIdList><ArticleId IdType="pubmed">25319679</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2014 Jul;203(2):535-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24750093</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2006 Aug;173(4):2371-81</Citation><ArticleIdList><ArticleId IdType="pubmed">16783025</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Popul Biol. 2016 Feb;107:65-76</Citation><ArticleIdList><ArticleId IdType="pubmed">26482676</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011 Feb 03;6(2):e16724</Citation><ArticleIdList><ArticleId IdType="pubmed">21304819</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2010 Oct;188(2):515-32</Citation><ArticleIdList><ArticleId IdType="pubmed">20831625</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 May 22;109(21):8264-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22570495</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2010 Apr;23(4):473-84</Citation><ArticleIdList><ArticleId IdType="pubmed">20192834</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2016 Feb;209(3):1240-51</Citation><ArticleIdList><ArticleId IdType="pubmed">26372471</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2006 Nov;18(11):3047-57</Citation><ArticleIdList><ArticleId IdType="pubmed">17085683</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Jan;197(1):162-76</Citation><ArticleIdList><ArticleId IdType="pubmed">23157484</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2016 Mar;202(3):1185-200</Citation><ArticleIdList><ArticleId IdType="pubmed">26721855</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Hum Genet. 2007 Sep;81(3):559-75</Citation><ArticleIdList><ArticleId IdType="pubmed">17701901</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2009 Nov;151(3):1667-76</Citation><ArticleIdList><ArticleId IdType="pubmed">19726571</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2017 Jan;213(2):799-811</Citation><ArticleIdList><ArticleId IdType="pubmed">27596807</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2016 Feb 24;16:49</Citation><ArticleIdList><ArticleId IdType="pubmed">26912051</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2009 Aug 15;25(16):2078-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19505943</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Rep. 2013 Dec 12;5(5):1279-93</Citation><ArticleIdList><ArticleId IdType="pubmed">24332370</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1995 Feb;139(2):963-73</Citation><ArticleIdList><ArticleId IdType="pubmed">7713445</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2015 Jan 23;16:24</Citation><ArticleIdList><ArticleId IdType="pubmed">25613058</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Chili</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Guerra, Fernando P" sort="Guerra, Fernando P" uniqKey="Guerra F" first="Fernando P" last="Guerra">Fernando P. 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